IMPACT OF THE USE OF YEASTS Saccharomyces cerevisiae AND NON-Saccharomyces β-Glucosidase-PRODUCING ON THE AROMATIC PROFILE OF WINES
β-glucosidase, aroma, wine, native yeasts.
The final quality of the wine depends on several factors, such as grape management practices, oenological technology and starter cultures used. The use of inoculated mixed cultures, using strains of S. cerevisiae and non-Saccharomyces, has been proposed as a solution to obtain the benefits of spontaneous fermentation without the risks of deterioration and/or fermentation arrest. The use of autochthonous yeasts producing β-glucosidase adds typicity to the wine, in addition to contributing to the release of volatile compounds, giving aromatic complexity to the product. The objective of this study was to characterize and select yeasts from the Collection of Microorganisms of Agroindustrial Interest (CMIA) that produce β-glucosidase and to evaluate their influence on the aromatic profile of the wines. A qualitative evaluation will be performed using the substrates arbutin, esculin and cellobiose, identifying the yeasts that produce β-glucosidase. The enzymatic activity will then be quantified using (p-NPG). Experimental wines will be produced with single and mixed inoculation, using native yeasts that produce β-glucosidase. For the selected strains of the species Saccharomyces cerevisiae, the high fermentative capacity will also be the subject of selection. The influence of the use of yeasts on the aromatic profile of the wines produced will be evaluated by sensory analysis and Gas Chromatography coupled to a mass spectrometer (GC-MS).