Banca de QUALIFICAÇÃO: CINTIA NEITZKE BECKER

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CINTIA NEITZKE BECKER
DATE: 07/04/2025
TIME: 08:30
LOCAL: Sala virtual meet.google.com/mnv-weeq-naw
TITLE:

ESSENTIAL OILS IN THE CONTROL OF FUNGI RPEVINE TRUNK DISEASE ASSOCIATED 

KEY WORDS:

Grapevine trunk disease; essential oils; biological fungicide

PAGES: 51
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitossanidade
SPECIALTY: Fitopatologia
SUMMARY:

Grapevine Trunk Diseases (GTDs) are a complex of fungal diseases whose gateway for the pathogens involved is lesions caused by pruning injuries or arising from routine cultural treatments. These diseases establish themselves in the plant's wood, attacking the plant's vascular system, thus causing a series of internal and external symptoms that lead the plant to decline and subsequent death. Among the main GTDs, and their causative pathogens are: Botryosphaeria dieback (Botryosphaeria spp., Lasiodiplodia spp. and Neofusicoccum spp.), Eutypiosis, or Eutypa Decline (Eutypa lata), Black Foot (Ilyonectria spp.), Petri's Disease and Esca (Phaeomoniella chlamydospora and Phaeoacremonium spp.).The lack of specific and truly effective treatments for the problem points to the need to search for alternatives that are economically viable, ecologically safe and truly efficient. In this context, the essential oils of Rosmarinus officinalis and Origanum vulgare have emerged, which have already been tested on other important fungal diseases with encouraging results. The present study aims to test the essential oils of Rosmarinus officinalis and Origanum vulgare in different concentrations, in order to determine the ideal application dose of the final product, which will be used to protect pruning wounds. In the initial test, doses of 1%, 1.5% and 2% of both essential oils were used. The Rosmarinus officinalis essential oil was incorporated into the BDA medium at room temperature (45°C) and subsequently poured into petri dishes where mycelium disks of the fungus Ilyonectria liriodendri, 70 mm in diameter, previously cultivated for 10 days also in PDA medium, were inoculated. The procedure was performed in the same way with oregano essential oil. Both assays were maintained in BOD at a temperature of 26°C for thirty days. The evaluation was performed daily by measuring the growth of the fungus on plates in both the horizontal and vertical directions. None of the treatments showed mycelial growth. Both oregano essential oil and rosemary essential oil inhibited 100% of the fungus Ilyonectria liriodendron at all doses tested. The second assay was performed using the same methodology with the fungi Ilyonectria liriodendri,Botryosphaeria dothidea, Neofusicoccum parvum and Phaeomoniella chlamydospora in direct confrontation with Rosmarinus officinalis and Origanum vulgare essential oils at concentrations of 0.5%, 0.25% and 0.125%. At these concentrations, Oreganum vulgare  essential oil showed excellent results, completely inhibiting mycelial growth in the fungi Botryosphaeria dothidea,Neofusicoccum parvum and Phaeomoniella chlamydospora. In the fungus Ilyonectria liriodendri, Origanum vulgare  essential oil completely inhibited the development of the fungus at concentrations of 0.5 and 0.25%. In addition to the results obtained, which are already really encouraging, the antioxidant potential of Rosmarinus officinalis and Origanum vulgare essential oils will also be tested using the DPPH method and the analysis of total phenols. With the final results, we will have the necessary information to formulate a biological product with curative/preventive power that does not present any risk to the producer or to the environment.

COMMITTEE MEMBERS:
Presidente - MARCUS ANDRE KURTZ ALMANCA
Interna - ROBERTA SCHMATZ
Interno - LUIS CARLOS DIEL RUPP
Externo à Instituição - Alexandre Meneghello Fuentefria